The Radnoti Porcine Working Heart System 140101EZ permits the researcher to create both constant pressure and constant flow re-circulating and non-re-circulating Langendorff preparations, as well as the working heart preparation for Large Hearts with high flow rates such as Porcine.  Due to the large scale of the apparatus and the donor heart, instrumentation is easily accommodated.

This version of the working heart system uses a peristaltic pump to generate the hydrostatic head for the constant pressure Langendorff preparation that initially sustains the heart.  Perfusate is drawn from the High Volume 5-liter water jacketed reservoir, routed through the atrial bubble trap and diverted up to the aortic bubble trap at the atrial cannula.  After the heart has stabilized, the researcher changes the direction of flow via a set of tubing clamps at “Y” connectors acting as a 3-way valve at the atrial cannula to switch from retrograde to working heart mode by directing perfusate into the left atria under hydrostatic pressure (5 mm Hg nominal, adjustable). The perfusate then enters the left ventricle and is pumped by the heart against an adjustable pressure head set by the researcher. After load may be adjusted on the aortic line to vary resistance by incorporating the supplied compliance loop on the aortic side during working heart mode. Buffer may be directed to waste following the compliance loop or directed to the 140144 Large Volume  Sheet Flow Oxygenating chamber for re-gassing and returned to the heart.  At this point the main 5-liter High Flow buffer reservoir supply may be omitted from the circuit and a nominal fixed volume (set by the researcher) re-circulated through the system.

The benefit of a pump fed compliance chamber used to generate the constant pressure Langendorff is that the researcher does not have to reposition elevated supply reservoirs. The heart chamber, components, and key perfusate lines are water-jacketed for superb temperature control.

The system may be purchased with or without data acquisition packages depending on your current laboratory needs. Also available in a No-Pump configuration 140101EZ-NP for users with access to existing equipment.

As with all Radnoti systems the, modular design allows for extreme flexibility and system reconfigurations.

The system ships complete with assembly manual and interactive basic principals of operation CD.