For experimentation requiring a sealed atmosphere such as hypoxia Radnoti has developed the atmospheric control tissue organ bath.  An o-ring sealed lid assembly with a center capillary for passage of a suture or fine stainless steel wire from the transducer to the sample, a lower hook or L-shape support, a gas vent with one way check valve and an septa port for sampling or dosing the inner chamber.  All passages through the lid assembly have dual o-ring seals to ensure a leak free fit.  A unique feature of the lid assembly is a press fit stainless steel pin that provides leverage for the user when removing the lid from the chamber.  A 3/8” aluminum rod slides over the stainless steel pin and locks into place via a thumb screw. With the aluminum rod in place the user can clamp the lid assembly to the stand and over a preparatory tissue bath for mounting of the sample.  Once mounted the entire lid assembly is then placed back into the tissue bath.

The special tissue bath is configured with all ports to the rear except the buffer in port.  The buffer in port is kept to the front of the chamber to allow easy access to the provided stopcock to fill the chamber.

An additional port has been added to this chamber to allow placement of an ion selective electrode for monitoring gas tensions with in the chamber. The electrode port is also placed to the rear of the chamber to reduce the chance of breakage.

The Atmospheric Control Tissue Organ Bath is available in both 10ml and 25ml bath volume configurations.