In
thin layer chromatography, there
is a frequent problem in recovery
of small samples for the plate.
The TLC Zone recovery pipette is
designed specifically for that
purpose. Its design reduces environmental
contamination due to silica dust
or materials such as radioisotopes
and toxins that are being separated
on the TLC plates, as well as increasing
the yield of isolated materials.
This instrument consists of a glass shaft with a vacuum hose
connection at the end (tube length 8mm x 150mm long). The front
of the shaft widens to a 13mm threaded neck. In the neck there
is a recessed, removable porous glass disc to prevent the sample
from exhausting into the vacuum line and to support a 10mm
filter membrane. The membrane porosity is at the user’s discretion,
but should not block the flow of air.
A polypropylene collar fastens a sturdy glass tip to the shaft
to complete the assembly. The principle of operation is the
same as a household vacuum cleaner. By scraping the spot or
zone on the plate with the tip, the particles are loosened
and then sucked up into the pipette due to the passage of air
into the vacuum hose.
The filter serves to stop particulates from exhausting in
a manner similar to the action of a household vacuum bag in
trapping dust. To remove the sample from the pipette, open
the collar, remove the tip and then remove the particulates
that have been trapped by the membrane filter or the removable
porous glass disc. You may also remove the vacuum hose and
back wash the pipette to remove the sample. Construction of
heavy-duty borosilicate glass assures sample purity. Each set
includes one pipette complete with three interchangeable frits.