Radnoti Blood Vessel Perfusion System

Measurements of isolated blood vessel contractility can be made on ring segments using isometric (or Isotonic) force transducers. Contractility measurements can also be made on blood vessel sections that have been cannulated and then are perfused at either a constant pressure or a constant flow. Radnoti has constructed a four-bath system that permits this type of measurement to be made conveniently.

The vessel section is cannulated with one of a set of cannulae and placed in a water-jacketed bath that can be aerated. In the constant pressure mode, a reservoir (or bubble trap) is placed above the bath at a given hydrostatic head and flow is changed by the contraction or dilation of the vessel. Flow measurements are made using drop counters, flow meters or other devices. In the constant flow mode, a peristaltic or syringe pump delivers fluid at a predetermined rate. In-line pressure transducers measure changes in diameter via changes in pressure.

In the constant flow system, drugs may be infused into the system via syringe pumps. In both modes, the media outside the vessels can be changed independently of the media perfused through the vessels. It is also possible to directly measure the diameter of the vessel using a camera. Note that in certain cases an aquarium pump can be used to aerate tissues with low oxygen requirements in non-bicarbonate buffer systems, thereby eliminating the problems and expense of gas tanks and regulators.

Instrumentation includes 4 pressure transducers, a peristaltic pump, reservoirs, bubble traps, recirculating bath, and all associated tubing and connectors. Available options: Indwelling electrodes for tissue stimulation, a tissue stimulator, flowmeters, or other fluid measuring devices, camera, air pump, and data acquisition with automation.

 

Part#	Description
158730	Glass Chamber and Head	4
159905	Pressure Transducer and Amplifier	4
158733-00	BVP Cannula (Straight) Set of 5 1.0mm, 1.5mm, 2.0mm, 2.5mm, 3.0mm	4
158734-00	BVP Cannula (Hooked) Set of 5 1.0mm, 1.5mm, 2.0mm, 2.5mm, 3.0mm	4
158728	Replacement Electrode Set	4
159952-11	Stand Clamp, Medium, f/RC & XDCR AMP	12
159952-10	Stand Clamp, Large F/Bar & DBL RC	8
130155-48	Water Jacketed Flex tube Assembly 48	8
120142-2	2-Liter Water Jacketed Reservoir	1
140144-2	Oxy Bubbler 2/Inlet for 2- Liter Reservoir	1
120143-3	Ring Clamp for 2-Liter Reservoir	1
120142-5	5-Liter Water Jacketed Reservoir	1
140144-5	Oxy Bubbler 2/Inlet for 5 Liter Reservoir	1
120143-5	Ring Clamp for 5-Liter Reservoir	1
159948	Reservoir Stand Assembly	1
120161	5-Port QD Manifold + Fittings to Allow Luer Lock	2
120165	5-Port Luer Manifold	1
120161	5-Port QD Manifold	2
120140-A	Tubing Adapter Kit	1
120142V001P	Plastic Vacuum Reservoir W/Lid and Fittings	1
158729	Replacement Addition Tube	4
159953-25	Ring Clamp 40-58mm Diameter	4
130149	Bubble Trap, QD	4
170051A	Bath/Heater Circulating Pump	1
170100A	Peristaltic Pump Drive	1
170110	Easy Load Pump Head w/hardware	2
170124-BVP	Four-Bar Stand for Blood Vessel Perfusion System	1
MISC	Tubing Kit for 4-Channel BVP	1
Manual	Assembly Manual	1