Isolated tissue preparations or tissue baths have been in use for over one hundred years, providing researchers with convenient biological models that exist without the systemic influences of the intact animal.
Additionally, isolated tissue preparations can generally be run in groups of 2,4, 8 or more, are more readily instrumented and can be more easily subjected to controlled changes in perfusate, oxygen, drugs and other factors than intact animals. The development of transgenic animal models has extended the scope of isolated tissue and organ preparations by creating models that can express normal or pathological human or non-host animal genetic sequences. In turn, isolated preparations can permit molecular biologists to quantitative the physiological impact of the expression of these altered genetic sequences at the tissue and organ level.

The Radnoti Tissue Bath Systems were created to enhance the productivity of researchers by permitting them to simultaneously monitor multiple preparations, allowing rapid replication of protocols. These systems give the researcher the option of a full range of different size baths in the same unit. The Radnoti tissue baths are individually mounted via adjustable ring-clamps to stainless steel support rods anchored to a sturdy steel base that is coated to resist the corrosive salt solutions often associated with such experiments.

The Tissue Bath Solution flows from a water jacketed, temperature controlled 2-liter reservoir into the glass manifold system that then distributes solution to the individual baths. Optional additional reservoirs may be purchased and added to perform complex drug additions and or wash out protocols. When the perfusate solutions enters the tissue baths solution passes through a pre-heating coil built within the water jacket of each High Tech tissue baths or via a 3-way stopcock at the drain in the 1583 series tissue organ baths.

The experimenter may empty the baths via a bottom drain controlled with a stopcock. The bath may also be used in a constant flow mode with the waste perfusate exiting an overflow outlet at the top of the bath. Both overflow and drains may be routed to permit monitoring the release of endogenous substances, drug metabolites, or to design a cascade system configuration. Bath aeration is finely controlled through the removable individual needle valves in each bath.

Complete with Radnoti 159901A Isometric Force Transducers (0-2gm, 0-20gm).
The 0-5 volt transducer output is conveniently shown on a full scale LED display on the amplifiers mounted above each chamber.

Each tissue bath has its own Isometric force transducer, transducer amplifier and tissue tensioner, permitting rapid adjustment and positioning of the specimen.

The systems are recommended for passive and stimulated muscle preparations using protocols including drug titrations, drug metabolism and kinetics, radio labeling, washout experiments, muscle force, and stress measurements for cardiac muscle, skeletal muscle, smooth muscle such as aortic rings, bladder or intestine as well as many other experiments. Thin, non-muscle preparations can be maintained in these baths as well. Systems are available in complete turnkey configurations or by individual components as with all of the extremely flexible Radnoti designs.

Tissue Organ System