Multi Channel Perfusion System

Radnoti has created a line of multi-channel perfusion systems for single cell and small tissue bath preparations, often used for electrophysiological studies and drug screening. Researchers have used luer lock syringes with tubing run to the receiving bath, but there are a number of problems with that approach. Because of the small volumes within the receiving baths, temperature and aeration can be greatly altered when bath solutions are replaced. Commercial systems that heat the solutions just prior to entry may have problems with solutions outgassing or be limited in terms of their inability to stability and reproducibly heat fluids that pass rapidly through their heaters. Most currently available commercial systems also cannot aerate the incoming bath solution.
The Radnoti systems are designed to maintain a perfusion solution’s temperature and aeration through the use of a water-jacketed reservoir and the use of either a flex-tube assembly (180102 System) or a heat exchanger (180102 System). Aeration is controlled via manifolds with individual aeration controls. The system can be adjusted to permit different gases to be used for hypoxia/ischemia or volatile anesthetic experiments. Water-jacketed systems are often more stable than small, electrically heated perfusate or syringe warmers because their reserve heat capacity is much larger, thereby reducing temperature fluctuations. The 180102 flex-tube system maintains the perfusate temperature constant through the length or the tubing run from the reservoir to the receiving bath. The 180101 heat exchanger system readjusts the perfusate temperature just before it enters the receiving bath and can be used in situations where supply line flexibility is critical. 180103 Micro Perfusion System is useful for systems requiring fluid delivery via peristaltic pump. All systems permit the use of reservoirs with volumes from 10 ml to 5 liters and fluids can be added during experimental runs through the use of the optional reservoir oxy bubbler with fluid return port.  The Radnoti 180102 and 180103 systems come standard with 3/32 x 5/32 tygon tubing.  Polyethylene or Teflon tubing in sizes ranging from 1/32 in and 1/16 in, the standards for many preparations, to larger tubing for faster flows and micro-bore tubing for smaller volumes are all available as options. The 180102 system uses the water jacketed Teflon tubing with a 1.8mm bore for the main perfusate run.  The systems can be controlled using optional solenoid valve and peristaltic pump systems, allowing for complex delivery regimens to be initiated via optional computer based control system or the user’s pre-existing equipment.