M Series Wire Myographs

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Radnoti Wire Myograph System

Designed for researchers preforming contractile force studies on small ring samples ranging from 60 μm to over 1 mm diameter and segment lengths up to 3mm. The Radnoti Wire Myograph is available in both four channel (M4) and single channel (M1) configurations. Its high quality fully modular design has been fine tuned to meet the needs of researchers in the field myography.

• Our M4 4 channel system now comes complete with Digital microscpe and monitor as standard! The microscope can be positioned over the low profile support stand to aid in visualization of the sample during mounting procedures.

• We provide extremely precise independent isometric force transducers based on picofarad capacitance converted to DC voltage.

• Safety lock for the ultra sensitive force transducer. A unique feature which gives an added margin of safety against expensive transducer damage whilst mounting the sample.

• Better calibration. Radnoti have developed a method for calibrating the myograph chamber in the horizontal position

• Due to its fully modular design each of the systems parts are independant, which allows for ease of repair without bringing research to a halt.

• Enhanced view of the sample due to inbeded light source in the 5ml chamber.

• Designed with researchers ease and comfort in mind. Ergonomic wrist holder allows for easy manipulation of the chambers and helps steady hands during use.

• Superb temperature control due to our independent Quick Start temperature controllers.

• Sturdy design: Steel base is coated to resist the corrosive salt solutions often associated with such experiments.

• Easy to clean and replace buffer inlet, gas inlet and buffer outlet tubes. The tubes are fabricated of borosilicate glass to give the researcher a clear view of the fluid path during setup and experimentation.

• Compatible with all leading data acquisition and software manufactures.

Overview of Radnoti Wire Myograph

When mounting the vessel segment, two individual parallel wires are passed through the lumen of the sample and attached to the micro vessel support brackets. Each vessel support bracket incorporates a micro groove on the face to aid in maintaining the wire position when mounting. The wires are then anchored via a locking screw on each of the brackets. (The locking screws feature a small bushing under the screw head that greatly improves capture of the support wire).

One support bracket is connected to a precision capacitance type isometric force transducer (0 – 0.5gm/0-5 gm, also available 0-2 gm/0-20 gm) that provides a true isometric measurement, i.e. less than 12.5μm deflection at 0.5gm force. This is critical when working with small vessel samples. The transducer amplifiers with LCD display are mounted directly above each myograph chamber. This is of particular benefit during application of sample pre-load, equilibration and length tension curves (Normalization procedures). The opposing support bracket is connected to a precision micrometer controlling the X axis movement, with Y and minor Z axis positioning controls conveniently located on the Myograph chamber, allowing for precise alignment of the micro vessel support brackets.

Once mounted into the 5ml chamber release the transducer safety lock and, using the X axis micrometer, rotate until desired pre-load is achieved. Repeat as required during equilibration period. Once optimal preload has been determined your experimental investigation can begin.

The Myograph system can be used to investigate:

• Small vessel length, diameter, function, wall tension and thickness.

• Isometric response to chemicals and pharmaceutical drugs.

• Endothelium studies.

• Role of ion channels, such as Calcium and Potassium, in smooth muscle.

• Contractile properties of small arterial resistance vessels.

These are just a few examples of the Wire Myographs many possible applications. With the M4 4-channel system researcher have the advantage of comparing samples against their controlls and under a variety of different conditions, which can be very benifical when studying the effects of drugs on small samples of smooth muscle.

*For samples with lumen diameters of 1mm or more, the standard Radnoti Tissue Organ Bath systems are recommended.