Radnoti Working Heart System #130101BEZ
- #130101BEZ is suitable for both mouse and rat
- Supports all Langendorff Modes in addition to Working Heart
- Atrial compliance loop: simulates physiological preload
- Afterload compliance loop: simulates physiological afterload and mimics normal vascular elasticity.
- Independently adjustable preload and afterload pressure
- Preparations may be spontaneously beating or paced using Radnoti #140157 Pacing Electrode
- Superb temperature control due to our water-jacketed heart chamber, components and water-jacketed key perfusate line tubing. Start-up to 37°C in 15 mins with less than 0.01° C error.
- Quick and easy disassembly due to our quick-disconnect tubing.
- Easy system maintenance and long-term durability due to GLP-compliant open architecture and no moving/active parts on compliance loops.
- Easy cleaning: our crystal clear borosilicate glassware is fully autoclavable and unlike plastic systems, are not affected by agents and proteins.
- Easy replacement: Individual components allow for easy replacement in the event of breakage and can be replaced without factory return.
- Customized configurations available. As with all Radnoti systems, the modular design allows for extreme flexibility and system reconfigurations.
The system can be instrumented to measure:
* LVP * Perfusate Flow * Ejection Flow
* Perfusate Pressure * Cardiac Output * ECG
* Optical Imaging * Luminescence * Fluorescence
* Apical Force * Ions * Metabolism
* Cardiac Pacing
Overview of Working Heart System
This version of the working heart system uses a peristaltic pump to generate the hydrostatic head (60mm Hg nominal, adjustable) for the constant pressure Langendorff preparation that initially sustains the heart. Perfusate is drawn from the water jacketed reservoir, routed through the atrial bubble trap and diverted up to the aortic bubble trap at the atrial cannula. After the heart has stabilized, the researcher changes the state of the 3-way valve at the atrial cannula to switch from retrograde to working heart mode by directing perfusate into the left atria under hydrostatic pressure (5 mm Hg nominal, adjustable). The perfusate then enters the left ventricle and is pumped by the heart against an adjustable pressure head set by the researcher. After load may be adjusted on the aortic line to vary resistance by incorporating the supplied compliance loop on the aortic side during working heart mode. Buffer may be directed to waste following the compliance loop or directed to the 130144 Sheet Membrane Oxygenating chamber for re-gassing and returned to the heart. At this point the main 2-liter buffer reservoir supply may be omitted from the circuit and a nominal fixed volume (set by the researcher) re-circulated through the system.
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