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A myosin II ATPase inhibitor reduces force production, glucose transport, and phosphorylation of AMPK and TBC1D1 in electrically stimulated rat skeletal muscle.
| Date | 2nd Jul '12 |
| Author(s) | David R. Blair, Katsuhiko Funai, George G. Schweitzer, and Gregory D. Cartee. |
| Journal | Am J Physiol Endocrinol Metab, May 2009; 296: E993 - E1002. |
Method Extract | |
| ... ... muscles were mounted in a water-jacketed glass container connected to a temperature-controlled bath containing the same medium as in the preceding incubation step (buffer 1 ± BTS) warmed to 35°C. With the use of stainless steel clips, the distal end of the muscle was attached to a glass rod, and the proximal end was attached to a force transducer (Radnoti, Litchfield, CT) with a resting tension of 0.4 g as previously described (17). Resting muscles were mounted in the same apparatus at tension of 0.4 g for 5 min but were not stimulated ... ... | |
| Keywords | isometric force transducer, skeletal muscle |
Our Ref: 1849
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