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Gene plasticity in colonic circular smooth muscle cells underlies motility dysfunction in a model of postinfective IBS.
| Date | 2nd Jul '12 |
| Author(s) | Barun K. Choudhury, Xuan-Zheng Shi, and Sushil K. Sarna Am J Physiol Gastrointest Liver. |
| Journal | Physiol, Mar 2009; 296: G632 - G642. |
Method Extract | |
| ... ... opened along the mesenteric border, cleaned, and pinned flat in a Petri dish with Sylgard base in carbogenated Krebs solution (in mmol/L: 118 NaCl, 4.7 KCl, 2.5 CaC2, 1 NaH2PO4, 1.2 MgCl2, 11 D-glucose, and 25 NaHCO3). The mucosal/submucosal layers were separated and discarded by microdissection. Circular muscle strips (4 mm x 10 mm) were mounted in individual muscle baths (Radnoti Glass, Monrovia, CA) filled with 5 ml carbogenated Krebs solution ... ... | |
| Keywords | colonic circular smooth muscle cells, tissue baths, contractility |
Our Ref: 1874
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