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Tetrahydrobiopterin (BH4), a cofactor for nNOS, restores gastric emptying and nNOS expression in female diabetic rats.

Date 2nd Jul '12
Author(s) Pandu R. R. Gangula, Sutapa Mukhopadhyay, Kalpana Ravella, Shijie Cai, Keith M. Channon, Robert E. Garfield, and Pankaj J. Pasricha.
Journal Am J Physiol Gastrointest Liver Physiol, May 2010; 298: G692 - G699.

Method Extract

... ...were mounted in 5-ml water-jacketed organ baths containing Krebs buffer at 37C and continuously bubbled with 95% O2-5% CO2 (Radnoti Glass Technology, Monrovia, CA). Tension for each muscle strip was monitored with an isometric force transducer and analyzed... ...

Keywords tissue bath, isometric force transducer, detrusor smooth muscle
Our Ref: 1889